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1.
Braz. j. oral sci ; 21: e226415, jan.-dez. 2022. tab
Article in English | LILACS, BBO | ID: biblio-1354995

ABSTRACT

Aim: Facial orthopaedic treatments based on the stimulation or restrictions of craniofacial bone growth are more effective when carried out during the pubertal growth spurt. The aim of this cross-sectional study was to evaluate the reproducibility of two cervical vertebrae methods (CVM) with manual tracing and direct visual inspection. Methods: A sample of 60 lateral cephalometric radiographs (10 of each of the 6 CVM stages) was randomly selected from 171 records. 5 orthodontists classified these radiographs according to the skeletal maturation stage in 2002 and 2005, and the application of both methods was conducted by direct visual inspection and evaluation through manual tracing. Results: The average reliability of the two methods determination and the two forms of evaluation was substantial. The direct visual inspection evaluation showed the highest reliability and agreement interexaminer values for both methods, as well as the intraexaminers evaluation. Conclusion: The reproducibility of CVM method was substantial, indicating its clinical use to determine the skeletal maturity and the ideal moment for treatment execution


Subject(s)
Bone Development , Cervical Vertebrae , Reproducibility of Results
2.
Rev. Fac. Odontol. Porto Alegre (Online) ; 62(1): 108-120, jan.-jun. 2021.
Article in Portuguese | LILACS, BBO | ID: biblio-1445001

ABSTRACT

Introdução: A indicação de higiene bucal em bebês edêntulos entre os profissionais de saúde ainda é controversa, sendo necessária a busca científica sobre esta indicação e a padronização da informação. Objetivo: Investigar criticamente as evidências relacionadas a indicação da higiene bucal para bebês edêntulos. Métodos: A busca de artigos foi realizada nas bases de dados PubMed, LILACS e Google Scholar, de modo a avaliar a seguinte pergunta: "A higienização da cavidade bucal de bebês edêntulos pode influenciar a microbiota bucal?". Uma busca em 8 livros de Odontopediatria também foi realizada. Resultados: Foram encontrados 317 artigos (167-PubMed, 146-Google Scholar e 4-LILACS). Não foram encontrados estudos primários que avaliaram o efeito da higiene bucal em bebês edêntulos na microbiota, impossibilitando a condução de revisão sistemática. Assim, para a revisão foram incluídos 6 estudos que investigaram micro-organismos na cavidade bucal de bebês edêntulos e o papel das imunoglobulinas salivares. Dentre os livros avaliados, somente 4 indicaram a higiene bucal em bebês edêntulos. Conclusão: Diante dos dados analisados, não existem estudos primários que avaliaram o efeito da higienização na microbiota bucal de bebês edêntulos. Torna-se relevante a condução de estudos clínicos para obtenção de evidências científicas sobre a indicação ou não da higienização da cavidade bucal de bebês edêntulos.


Introduction: The indication of oral hygiene in edentulous babies is still controversial among health professionals, being necessary the search of this recommendation and the standardization of information. Objective: To inves-tigate critically the evidence related to the indication of oral hygiene for edentulous babies. Methods: The search for articles was performed in the PubMed, LILACS and Google Scholar databases, in order to assess the following question: "Can oral cavity hygiene of edentulous babies influence oral microbiota?" A search in 8 books of Pediatric Dentistry was also performed. Results: 317 articles were found (167-PubMed, 146-Google Scholar and 4-LILACS). There were no primary studies that evalu-ated the effect of oral hygiene of edentulous infants on microbiota, which impair the conduction of a systematic review. Thus, it was included for this review six studies that investigated microorganisms in the oral cavity of edentulous infants and the role of salivary immunoglo- bulins. Among the textbooks evaluated, only 4 indicated the oral hygiene in edentulous infants. Conclusion:According to the data, there are no primary studies that assessed the effect of oral hygiene in the oral microbiota of edentulous babies. It is relevant to conduct clinical studies in order to obtain scientific evidence about the indication or no of the oral hygiene in edentulous babies


Subject(s)
Humans , Infant, Newborn , Infant , Oral Hygiene , Mouth, Edentulous , Microbiota
3.
J. appl. oral sci ; 29: e20210120, 2021. tab, graf
Article in English | LILACS | ID: biblio-1340104

ABSTRACT

Abstract Objective Our study aims to synthesize, characterize, and determine the effects of a ChNPs suspension on human enamel after cariogenic challenge via pH-cycling. Methodology ChNPs were synthesized by ion gelation and characterized by Transmission Electron Microscopy (TEM) and Dynamic Light Scattering. Forty enamel blocks were divided into four groups (n=10/group): (i) ChNPs suspension; (ii) chitosan solution; (iii) 0.05% sodium fluoride (NaF) solution; and (iv) distilled water. Specimens were exposed to cariogenic challenge by cycling in demineralization solution (3 h) and then remineralized (21h) for 7 days. Before each demineralization cycle, the corresponding solutions were passively applied for 90 s. After 7 days, specimens were examined for surface roughness (Ra) and Knoop hardness (KHN) before and after the cariogenic challenge; % KHN change (variation between initial and final hardness), and surface topography by an optical profilometer. The data were analyzed by repeated-measures ANOVA, One-way ANOVA, and Tukey tests (α=0.05). Results TEM images showed small spherical particles with diameter and zeta potential values of 79.3 nm and +47.9 mV, respectively. After the challenge, all groups showed an increase in Ra and a decrease in KHN values. Optical profilometry indicated that ChNPs- and NaF-treated specimens showed uneven roughness interspersed with smooth areas and the lowest %KHN values. Conclusion The ChNPs suspension was successfully synthesized and minimized human enamel demineralization after a cariogenic challenge, showing an interesting potential for use as an oral formulation for caries prevention.


Subject(s)
Humans , Tooth Demineralization/prevention & control , Chitosan , Nanoparticles , Sodium Fluoride , Cariostatic Agents , Dental Enamel , Hardness
4.
Rev. odontol. UNESP (Online) ; 50: e20210020, 2021. tab, ilus
Article in English | LILACS, BBO | ID: biblio-1347770

ABSTRACT

Introduction: Enamel microabrasion is achieved by abrasion of the enamel surface and it is a quick, practical and efficient procedure. Objective It was assessed the weight loss and surface aspect of enamel after microabrasion procedures with commercial and mixtures made in-office. Material and method Eighty bovine incisors were divided into four groups (n=10): OpalustreTM (6.6% HCl + silicon carbide); Whiteness RMTM (6% HCl + silicon carbide); 37% H3PO4 + pumice and 10% HCl + pumice. Treatment was performed by 15 applications of 10s duration. The enamel weight loss was determined by the difference in weight before and after the microabrasion. The surfaces were analyzed by a surface roughness equipment and scanning electron microscopy. Data were submitted to paired-T test, one-way ANOVA and Tukey tests (α=0.05). Result It was observed significantly weight loss for all groups. The difference in weight loss ranged from 0.037±0.012 for group 37% H3PO4 and from 0.054±0.009 for group 6,6% HCl. There was a significant increase in surface roughness for all groups and 10% HCl group showed the lower results (0.65±0.09). It was observed different patterns of enamel morphology by SEM images. Conclusion The in-office mixtures resulted in the lowest enamel structure loss (37% H3PO4) and the lowest surface roughness (10% HCl).


Introdução: A microabrasão do esmalte é realizada através da abrasão da superfície do mesmo, apresentando-se como um procedimento rápido, prático e eficiente. Objetivo Avaliar a perda de peso e o aspecto superficial do esmalte após procedimentos de microabrasão com agentes comerciais e não comerciais. Material e método Oitenta incisivos bovinos foram divididos em quatro grupos (n = 10): OpalustreTM (6,6% HCl + carboneto de silício); Whiteness RMTM (6% HCl + carboneto de silício); 37% H3PO4 + pedra-pomes e 10% HCl + pedra-pomes. O tratamento foi realizado em 15 aplicações de 10s de duração. A perda de peso do esmalte foi determinada pela diferença de peso antes e depois da microabrasão. As superfícies foram analisadas por equipamento de rugosidade superficial e microscopia eletrônica de varredura. Os dados foram submetidos ao teste T-pareado, ANOVA one-way e teste de Tukey (α = 0,05). Resultado Foi observada perda de peso significativa para todos os grupos, a diferença na perda de peso variou de -0,037±0,012 para o grupo 37% H3PO4 a -0.054±0.009 para o grupo 6.6% HCl. Houve um aumento significativo na rugosidade da superfície para todos os grupos e o grupo 10%HCl apresentou os resultados mais baixos (0,65±0,09). Foram observados diferentes padrões de morfologia do esmalte por meio de imagens MEV. Conclusão Os agentes não comerciais resultaram na menor perda de estrutura do esmalte (37% H3PO4) e na rugosidade superficial (10% HCl).


Subject(s)
Animals , Cattle , Microscopy, Electron, Scanning , Analysis of Variance , Enamel Microabrasion , Dental Enamel , Tooth Wear
5.
HU rev ; 44(1): 115-122, 2018.
Article in Portuguese | LILACS | ID: biblio-986573

ABSTRACT

Introdução: Diversos materiais restauradores são indicados para a restauração de dentes decíduos posteriores, como amálgama, resina composta, cimento de ionômero de vidro convencional, cimento de ionômero de vidro modificado por resina e resinas compostas modificadas com poliácidos (compômeros). Entretanto, uma dúvida ainda persiste quanto ao desempenho clínico do cimento de ionômero de vidro, em decorrência de suas propriedades, para ser utilizado como material restaurador definitivo em molares decíduos. Objetivo: Realizar uma revisão sistemática da literatura acerca da taxa de sobrevivência de restaurações realizadas em molares decíduos utilizando resina composta e cimento de ionômero de vidro, buscando com base em evidências científicas, responder a PICO question: "Qual material possui maior longevidade em molares decíduos: resina composta ou cimento de ionômero de vidro?". Material e métodos: Para este fim foram realizadas buscas em duas bases de dados, PubMed e Web of Science, utilizando uma estratégia de busca previamente determinada, para selecionar artigos de acordo com critérios de inclusão e exclusão criados para esse trabalho por dois examinadores calibrados. Resultados: A seleção dos artigos foi realizada de acordo com o fluxograma do PRISMA e foi criada uma tabela para avaliação qualitativa dos artigos selecionados. Foram encontrados 398 artigos na base de dados PubMed e 375 na Web of Science, sendo 153 coincidentes em ambas. Quarenta e seis artigos foram selecionados para leitura completa, dentre eles 42 foram excluídos, e quatro artigos foram incluídos para esta revisão. Conclusão: Os artigos desta revisão demonstraram que a resina composta possuiu maior longevidade em molares decíduos comparada aos cimentos de ionômero de vidro. Porém futuros estudos clínicos necessitam ser conduzidos para comprovar qual o material mais indicado para restaurá-los, já que os cimentos de ionômero modificados por resina mostraram taxas de sobrevivência clínica satisfatórias.


Introduction: Several restorative materials are indicated for restoration of posterior deciduous teeth, such as amalgam, composite resin, conventional glass ionomer cement, resin modified glass ionomer cement and composite resins modified with polyacids (compomers). However, a doubt still persists related to the clinical performance of the glass ionomer cements to be used to restore deciduous molars, due to its properties. Objective: To perform a systematic review of the literature about the survival rate of composite resin and glass ionomer cement restorations performed in deciduous molars aiming scientific evidence to answer the PICO question: "Which material has greater longevity in deciduous molars: resin composite or glass ionomer cement?" Material and methods: The research was performed on two databases, PubMed and Web of Science, using a predetermined search strategy to select articles according to the inclusion and exclusion criteria, evaluated by two calibrated examiners. Results: The selection of the articles was carried out according to the PRISMA flowchart and a table was created for the qualitative evaluation of the included articles. It was found 398 articles in the PubMed database and 375 in the Web of Science, 153 of which were coincident in both. Forty-six articles were selected for complete reading, 42 of which were excluded, and four articles were included in this review. Conclusion: The articles investigated in this review demonstrated that composite resin has a higher longevity in deciduous molars compared to glass ionomer cements. However, future clinical studies are needed to attest which material is better to restore them, since the resin modified glass ionomer cements showed satisfactory clinical survival rates.


Subject(s)
Tooth, Deciduous , Composite Resins , Survival Rate , Glass Ionomer Cements , Longevity , Molar
6.
J. appl. oral sci ; 26: e20170222, 2018. tab, graf
Article in English | LILACS, BBO | ID: biblio-893701

ABSTRACT

Abstract The effect of fluoride agents on the retention of orthodontic brackets to enamel under erosive challenge is little investigated. Objective: The aim of this study was to evaluate the effect of titanium tetrafluoride (TiF4) and sodium fluoride (NaF) agents on the shear bond strength of brackets to enamel and on the enamel microhardness around brackets under erosive challenge. Methods: Brackets were bonded to bovine incisors. Five groups were formed according to fluoride application (n=10): TiF4 varnish, TiF4 solution, NaF varnish, NaF solution and control (without application). The specimens were submitted to erosive challenge (90 s cola drink/2h artificial saliva, 4x per day for 7 days). Solutions were applied before each erosive cycle and varnishes were applied once. Vickers Microhardness (VHN) was obtained before and after all cycles of erosion and the percentage of microhardness loss was calculated. Shear bond strength, adhesive remnant index and polarized light microscopy were conducted after erosion. The data were analyzed by ANOVA, Tukey, Kruskal-Wallis and Mann-Whitney U tests (α=0.05). Results: The %VHN had no statistically significant differences among the experimental groups. However, considering the comparisons of all groups with the control group, TiF4 varnish showed the highest protection from enamel demineralization (effect size of 2.94, while the effect size for the other groups was >2.4). The TiF4 varnish group had significantly higher shear bond strength compared to other groups. There was no difference among groups for adhesive remnant index. Polarized light microscopy showed higher demineralization depth for the control group. Conclusions: Application of NaF and TiF4 agents during mild erosive challenge minimized the enamel mineral loss around brackets, however only the experimental TiF4 varnish was able to prevent the reduction of shear bond strength of brackets to enamel.


Subject(s)
Animals , Cattle , Sodium Fluoride/chemistry , Titanium/chemistry , Tooth Erosion/prevention & control , Cariostatic Agents/chemistry , Dental Bonding/methods , Orthodontic Brackets , Dental Enamel/drug effects , Fluorides/chemistry , Reference Values , Saliva, Artificial/chemistry , Surface Properties , Materials Testing , Reproducibility of Results , Analysis of Variance , Tooth Demineralization/prevention & control , Statistics, Nonparametric , Dental Enamel/chemistry , Shear Strength , Hardness Tests , Microscopy, Polarization
7.
Rev. odontol. UNESP (Online) ; 45(2): 103-109, mar.-abr. 2016. tab, ilus
Article in English | LILACS, BBO | ID: lil-780064

ABSTRACT

Introduction: Few studies investigated the surface properties of luting cements after erosive challenge. Objective: To evaluate the surface roughness (Ra), Vickers hardness (VHN) and morphology of 4 luting cements after erosive challenge. Material and method: Twenty specimens of each cement were prepared (4×2mm) and divided into experimental (erosive challenge) and control (artificial saliva) groups (n=10): Rely X U200 (U200); Rely X ARC (ARC); Ketac Cem Easy Mix (Ketac) and Zinc phosphate (ZnP). The erosive challenge was performed by four daily erosive cycles (90s) in a cola drink and 2 h in artificial saliva over 7 days. Ra and VHN readings were performed before and after erosion. The percentage of hardness loss (%VHN) was obtained after erosion. The surface morphology was analyzed by scanning electron microscopy (SEM). ANOVA, Tukey and Student-T tests were used (α=0.05). Result: After erosion, all luting cements had increase in Ra values and U200 and ZnP groups had the highest %VHN. After saliva immersion, only U200 and ZnP groups had significant increases in Ra values and there were no significant differences among the groups in %VHN. SEM analysis showed that Ketac and ZnP groups had rough and porous surfaces, and U200 group had higher resin matrix degradation than ARC group. Conclusion: Erosive challenge with a cola drink affected the surface properties of all luting cements.


Introdução: Poucos estudos investigaram as propriedades de superfície de cimentos após desafio erosivo. Objetivo: Avaliar a rugosidade da superfície (Ra), dureza Vickers (VHN) e morfologia de superfície de 4 cimentos após desafio erosivo. Material e método: Vinte amostras de cada cimento foram preparadas (4×2mm) e divididas em grupo experimental (desafio erosivo) e controle (saliva artificial) (n=10): Rely X U200 (U200); Rely X ARC (ARC); Ketac Cem Easy Mix (Ketac) e Fosfato de Zinco (ZnP). O desafio erosivo foi realizado com quatro ciclos erosivos diárias (90s) em bebida à base de cola e 2h em saliva artificial durante 7 dias. As leituras de Ra e VHN foram realizadas antes e após erosão. A porcentagem de perda de dureza (%VHN) foi obtida depois da erosão. A morfologia de superfície foi analisada por microscopia eletrônica de varredura (MEV). Foram utilizados testes de ANOVA, Tukey e T-Student (α=0,05). Resultado: Após a erosão, foi observado aumento dos valores de Ra em todos os cimentos testados, e os grupos U200 e ZNP tiveram a maior %VHN. Após imersão em saliva, apenas os grupos U200 e ZnP tiveram aumento significativo nos valores de Ra e não houve diferenças significativas entre os grupos quanto à %VHN. A análise em MEV mostrou que os grupos Ketac e ZNP apresentaram superfícies rugosas e porosas, e o grupo U200 apresentou maior degradação da matriz comparado ao grupo ARC. Conclusão: O desafio erosivo com bebida a base de cola afetou as propriedades de superfície de todos os cimentos.


Subject(s)
Tooth Erosion , Microscopy, Electron, Scanning , Analysis of Variance , Resin Cements , Glass Ionomer Cements , Hardness Tests , Saliva, Artificial , Surface Properties , Beverages
8.
Braz. j. oral sci ; 14(4): 276-281, Oct.-Dec. 2015. tab
Article in English | LILACS | ID: lil-778244

ABSTRACT

To assess in vitro the surface roughness (Ra), Vickers hardness (VHN) and surface morphology of resin and glass ionomer materials used for sealants after dynamic erosive challenge. Methods: Twenty specimens of each material were prepared and divided into experimental (erosive challenge) and control groups (n=10): Protect Riva (SDI), Opallis Flow (3M ESPE), Fluroshield (Dentsply), Filtek Z350 XT Flow (3M ESPE). The erosive challenge was performed 4 times per day (90 s) in cola drink and for 2 h in artificial saliva for 7 days. The control specimens were maintained in artificial saliva. Ra and VHN readings were performed before and after erosion. The percentage of hardness loss (%VHN) was obtained after erosion. The surface morphology was evaluated by scanning electron microscopy (SEM). The data were analyzed by ANOVA, Tukey and paired t tests (á=0.05). Results: After erosion and saliva immersion, there was an increase in Ra values for all groups and Riva group showed the highest Ra values. After erosive challenge, Riva and Filtek groups showed significant decrease in VHN values, but Filtek group showed the greatest %VHN. For all groups there was inorganic particle protrusion and matrix degradation after erosion visualized by SEM images. Conclusions: Erosive challenge affected the surface properties of all materials used as sealants, particularly in the Riva and Filtek groups...


Subject(s)
Humans , Composite Resins , Hardness Tests
9.
Dental press j. orthod. (Impr.) ; 19(5): 110-115, Sep-Oct/2014. tab, graf
Article in English | LILACS | ID: lil-727102

ABSTRACT

OBJECTIVE: To assess the level of knowledge of primary school teachers in the public school network of Northeastern Brazil with respect to management of dental trauma and its relationship with prognosis. METHODS: A questionnaire was applied to 195 school teachers of public schools in Northeastern Brazil. The questionnaire comprised 12 objective questions about dental trauma and methods for its prevention and management. Data were submitted to chi-square test and Poisson regression test (P > 0.05). RESULTS: Out of the 141 teachers who responded the questionnaires, the majority were women (70.2%) and most of them had experienced previous dental accidents involving a child (53.2%). The majority (84.4%) had incomplete college education and few were given some training on how to deal with emergency situations during their undergraduate course (13.5%) or after it (38.3%). Their level of knowledge about dental trauma and emergency protocols showed that unsatisfactory knowledge level was associated with the male sex: 46% higher for men in comparison to women (P = 0.025). CONCLUSIONS: Approximately half of teachers evaluated had unsatisfactory knowledge about dental trauma and emergency protocols, with female teachers showing more knowledge than men. .


OBJETIVO: avaliar o nível de conhecimento de professores de escolas primárias na rede pública de ensino na região nordeste do Brasil, no que diz respeito ao manejo do paciente traumatizado e sua relação com o prognóstico. MÉTODOS: aplicou-se um questionário a 195 professores da rede pública de ensino da região nordeste do Brasil. O questionário continha 12 questões objetivas sobre os traumatismos dentários e seus métodos de prevenção e manejo. Após obtenção dos dados, esses foram submetidos ao teste qui-quadradro e ao teste de regressão de Poisson (p > 0,05). RESULTADOS: dos 141 professores que responderam o questionário, a maioria era composta pelo sexo feminino (70,2%) e a maior parte já vivenciara algum episódio de acidente com criança (53,2%). A maioria (84,4%) possui ensino superior incompleto e poucos foram contemplados com manobras de urgência na graduação (13,5%) ou após essa (38,3%). Os resultados da regressão para o nível de conhecimento sobre traumatismos dentários e protocolo de urgência em relação às variáveis explanatórias do estudo demonstraram que o conhecimento insatisfatório foi associado ao sexo masculino, sendo 46% maior para os homens (p = 0,025). CONCLUSÃO: aproximadamente metade dos professores possui conhecimento insatisfatório sobre traumatismos dentários e protocolo de urgência, sendo que os professores do sexo feminino apresentaram maior conhecimento do que os do sexo masculino. .


Subject(s)
Adult , Child , Female , Humans , Male , Middle Aged , Faculty , First Aid/methods , Health Knowledge, Attitudes, Practice , Tooth Injuries/therapy , Accidents , Brazil , Educational Status , Health Education, Dental , Organ Preservation Solutions/therapeutic use , Public Sector , Schools , Sex Factors , Tooth Injuries/prevention & control , Tooth Replantation/methods
10.
Dental press j. orthod. (Impr.) ; 19(4): 66-70, Jul-Aug/2014. tab
Article in English | LILACS | ID: lil-725418

ABSTRACT

OBJECTIVE: The aim of this study was to assess the in vitro cytotoxicity of acrylic resins of different colors over time. METHODS: Specimens were divided into 4 groups (n = 6) according to the color of the acrylic resin (Orto Class, Clássico, Campinas, São Paulo, Brazil): Group 1: clear acrylic resin; group 2: pink acrylic resin; group 3: blue acrylic resin and group 4: green acrylic resin. All specimens were fabricated according to the mass manipulation technique and submitted to mechanical polishing protocol. The control was performed with an amalgam specimen (C+), a glass specimen (C-) and cell control (CC). Specimens were immersed in Minimum Eagle's Medium (MEM) and incubated for 24 h at 37o C. The extracts from the experimental material were filtered and mixed with L929 fibroblast. Cytotoxicity was evaluated at 4 different times, 24, 48, 72 and 168 h. After contact, cells were incubated for 24 h and added to 100 µ of 0.01% neutral red dye. The cells were incubated for 3 h for pigment incorporation and fixed. Cells viability was determined by a spectroscopic (BioTek, Winooski, Vermont, USA) with a 492-nm wavelength λ=492 nm). RESULTS: There were no statistical differences between the experimental groups and the CC and C- groups. CONCLUSION: Clear, pink, blue and green self-curing acrylic resins fabricated by means of the mass manipulation technique and mechanically polished are not cytotoxic. Neither the pigment added to the self-curing acrylic resin nor the factor of time influenced the cytotoxicity of the material. .


OBJETIVO: avaliar, in vitro, a citotoxicidade de resinas acrílicas autopolimerizáveis, de diferentes cores, ao longo do tempo. MÉTODOS: os corpos de prova foram divididos em quatro grupos (n = 3), de acordo com a cor da resina acrílica utilizada (Orto Class, Clássico, São Paulo/SP), sendo: grupo 1, acrílica incolor; grupo 2, acrílica rosa; grupo 3, acrílica azul; e, grupo 4, acrílico verde. Todos os corpos de prova foram confeccionados pela técnica de massa e polidos mecanicamente. Um corpo de prova de amálgama, um de vidro e célula constituíram o controle positivo (C+), controle negativo (C-), e controle de célula (CC), respectivamente. Em seguida, esses foram imersos em meio mínimo essencial de Eagle (MEM) por 24h, quando se removeu o sobrenadante e colocou-os em contato com fibroblastos L929. Avaliou-se a citotoxicidade em quatro períodos: 24, 48, 72 e 168h. Após o contato com o meio, as células foram incubadas por 24h e adicionou-se 100µ do corante vermelho neutro a 0,01%. Posteriormente, as células foram incubadas por 3h, para incorporação do corante, e fixadas. A contagem das células viáveis foi realizada em espectrofotômetro (BioTek, Winooski, EUA), com um comprimento de onda de 492nm (λ = 492nm). RESULTADOS: não houve diferença estatística entre os grupos experimentais e os grupos CC e C-. CONCLUSÇÕES: as resinas acrílicas autopolimerizáveis incolor, rosa, azul e verde, manipuladas pela técnica de massa e polidas mecanicamente não são citotóxicas. O corante utilizado em resinas autopolimerizáveis e tempo não influenciam na citotoxocidade do material. .


Subject(s)
Animals , Mice , Acrylic Resins/toxicity , Coloring Agents/toxicity , Dental Materials/toxicity , Cell Culture Techniques , Cell Line , Color , Cell Survival/drug effects , Dental Amalgam/toxicity , Dental Polishing/methods , Fibroblasts/drug effects , Glass/chemistry , Indicators and Reagents , Materials Testing , Neutral Red , Polymerization , Spectrum Analysis , Surface Properties , Self-Curing of Dental Resins/methods , Temperature , Time Factors
11.
Braz. dent. j ; 25(2): 170-174, Mar-Apr/2014. tab, graf
Article in English | LILACS | ID: lil-719222

ABSTRACT

The aim of this study was to evaluate the post-treatment anteroposterior and vertical alterations in skeletal Class II malocclusion with different maxillary patterns in patients treated with modified Thurow appliance. Forty-five patients (22 girls and 23 boys) with skeletal Class II and angle SN.GoGn ≤ 35 and different maxillary patterns (n=15), as follows: retrusive (SNA<80°), normal (SNA=80°- 84°) or protrusive (SNA>84°) maxilla; mean age 9 years at pre-treatment (T1) and 9 years and 10 months at post-treatment (T2), were treated with modified Thurow cervical traction appliance, with expander screw and extraoral face bow with 10° to 20° fold in relation to the intraoral arch. Force of 500 gf was applied and use for 12 to 14 h/day, with fortnightly adjustments. Analysis of variance ANOVA followed by post-hoc Tukey and Kruskal-Wallis test, followed by Mann-Whitney were used (α=5%). In changes obtained from stage T1 to T2, no statistically significant differences were found among the groups Protrusive, normal and retrusive maxilla for the variables SNB, SN.GoGn, 1.NA, overjet, overbite and Class II discrepancy (right and left) (p>0.05). Angular measurements SNA and ANB in the protrusive maxilla group were significantly greater than in the normal and retrusive maxilla groups (p<0.01). However, in the normal maxilla group these values did not differ significantly from those of the retrusive maxilla group (p>0.05). Within the limits of this study, it may be concluded that the modified Thurow cervical traction appliance was efficient for the correction of skeletal Class II irrespective of the maxillary pattern. The mandible had no significant rotation during treatment.


O objetivo deste estudo foi avaliar as alterações verticais e ântero-posterior pós-tratamento da maloclusão Classe II esquelética com diferentes padrões maxilar de pacientes tratados com aparelho Thurow modificado. Quarenta e cinco pacientes (22 meninas e 23 meninos) com Classe II esquelética e ângulo SN.GoGn≤35 e diferente padrão maxilar (n=15),sendo: maxila retrusiva (SNA<80°), normal (SNA= 80°-84°) ou protrusiva (SNA>84°) e idade média de 9 anos no pré-tratamento (T1) e 9 anos e 10 meses no pós-tratamento (T2), foram tratados com aparelho Thurow modificado de tração cervical com parafuso expansor e arco facial externo com dobra de 10° a 20° em relação ao arco interno. Foi empregado a força de 500 gf e uso de 12 a 14 h/dia, com ajustes quinzenais. Análise de variância ANOVA seguido pelo teste post-hoc de Tukey e o teste de Kruskal-Wallis seguido por Mann-Whitney foram empregados (p<0.05). Nas mudanças obtidas da fase T1 para T2, não foram encontradas diferenças estatísticas entre os grupos maxila protusiva, normal e retrusiva para as variáveis SNB, SN.GoGn, 1.NA, overjet, overbite e discrepância de classe II (direita e esquerda) (p>0.05). As medidas angulares SNA e ANB no grupo maxila protusiva foi significativamente maior do que nos grupos maxila normal e retrusiva (p<0.01). Entretanto, esses valores no grupo maxila normal não diferiram do grupo maxila retrusiva (p>0.05). Dentro dos limites do estudo, pode-se concluir que o aparelho de Thurow modificado de tração cervical foi eficiente na correção da Classe II esquelética independente do padrão maxilar. A mandíbula não teve rotação significativa durante o tratamento.


Subject(s)
Child , Humans , Extraoral Traction Appliances , Malocclusion, Angle Class II/therapy , Malocclusion, Angle Class II/pathology
12.
Braz. oral res ; 27(6): 463-470, Nov-Dec/2013. tab, graf
Article in English | LILACS | ID: lil-695990

ABSTRACT

The aim of this study was to assess the effect of different remineralizing agents on enamel microhardness (KHN) and surface topography after an erosive challenge. Forty-eight human enamel specimens (4 × 4 mm) were randomly assigned to 4 groups: control (no treatment), fluoride varnish, calcium nanophosphate paste and casein phosphopeptide-amorphous calcium phosphate paste (CPP-ACP). Both pastes were applied for 5 minutes, and fluoride varnish, for 24 h. Four daily erosive cycles of 5 minutes of immersion in a cola drink and 2 h in artificial saliva were conducted for 5 days. KHN readings were performed at baseline and after 5 days. The percentage of enamel hardness change (%KHN) was obtained after erosion. The surface topography was evaluated by atomic force microscopy (AFM). The data were tested using ANOVA, Tukey's and paired-T tests (p < 0.05). After an erosive challenge, there was no statistically significant difference between the control (96.8 ± 11.4 KHN / 72.4 ± 3.0 %KHN) and the varnish (91.7 ± 14.1 KHN / 73.4 ± 5.5 %KHN) groups. The nanophosphate group showed lower enamel hardness loss (187.2 ± 27.9 / 49.0 ± 7.9 %KHN), compared with the CPP-ACP group (141.8 ± 16.5 / 60.6 ± 4.0 %KHN), and both were statistically different from the varnish and the control groups. AFM images showed a rough surface for the control and the varnish groups, a non-homogeneous layer with globular irregularities for CPP-ACP, and a thick homogeneous layer for the nanophosphate group. None of the agents provided protection against the development of erosion; however, nanophosphate paste was able to reduce enamel surface softening after the erosive challenge.


Subject(s)
Humans , Calcium Phosphates/therapeutic use , Caseins/therapeutic use , Dental Enamel/drug effects , Tooth Erosion/prevention & control , Tooth Remineralization/methods , Analysis of Variance , Carbonated Beverages , Dental Enamel/chemistry , Hardness Tests , Microscopy, Atomic Force , Nanoparticles , Random Allocation , Reproducibility of Results , Saliva, Artificial/chemistry , Surface Properties/drug effects , Time Factors
13.
Braz. dent. j ; 24(5): 492-497, Sep-Oct/2013. tab
Article in English | LILACS | ID: lil-697633

ABSTRACT

This study evaluated the influence of the manipulation technique and polishing method on the flexural strength and cytotoxicity of acrylic resins. Two manipulation techniques and three polishing methods were used in the fabrication of acrylic plates that were divided into 6 groups (n=10). Groups MM, MC and MW: mass technique with mechanical polishing, chemical polishing and without polishing, respectively; and Groups SM, SC and SW: Saturation technique with mechanical polishing, chemical polishing and without polishing, respectively). Flexural strength was tested in a universal testing machine and the cytotoxicity assay used cell cultures (L-929) for periods of 24 h to 168 h. Flexural strength and cytotoxicity data were assessed using two-way and three-way ANOVA, respectively (α=0.05), followed by post hoc Bonferroni test for multiple comparisons. The effect of combinations of manipulation techniques and polishing methods on flexural strength showed significant differences only between Group SC and Groups MW, MM and MC (p<0.01). Cell viability ranged from 51% (3.9%) to 87,6% (3.2) in the 24-h time interval, and from 87.8% (5.0) to 95.7% (3.1%) in the 168-h time interval. With the increase of cell viability, from the third day (72 h), there was no significant difference among the groups, except between MM and SC (p<0.01) at 72 h. In conclusion, the manipulation technique and polishing method had more influence on the cytotoxicity than on flexural strength.


Este estudo avaliou a influência da técnica de manipulação e método de polimento sobre a resistência à flexão e citotoxicidade de resinas acrílicas. Duas técnicas de manipulação e três métodos de polimento foram usados na fabricação de placas de acrílico que foram divididas em 6 grupos (n=10). Grupos MM, MC e MW: técnica de massa com polimento mecânico, polimento químico e sem polimento, respectivamente; e Grupos SM, SC e SW: técnica de saturação com polimento mecânico, polimento químico e sem polimento, respectivamente. A resistência à flexão foi testada em uma máquina universal de ensaios e o ensaio de citotoxicidade foi realizada utilizando culturas de células (L929) para os períodos de 24 h a 168 h. Dados da resistência à flexão e de citotoxicidade foram avaliados usando ANOVA dois fatores e ANOVA três fatores, respectivamente (α=0,05), seguido pelo teste post hoc de Bonferroni para comparações múltiplas. O efeito das combinações de técnicas de manipulação e métodos de polimento na resistência à flexão mostraram diferenças significativas apenas entre Grupo SC e Grupos MW, MM e MC (p<0,01). A viabilidade celular variou de 51,0% (3,9%) para 87,6% (3,2%) no intervalo de tempo de 24 h, e de 87,8% (5,0%) para 95,7% (3,1%) no intervalo de tempo de 168 h. Com o aumento da viabilidade celular, a partir do terceiro dia (72 h), não houve diferença significativa entre os grupos, exceto entre MM e SC (p<0,01) em 72 h. Em conclusão, a técnica de manipulação e o método de polimento tiveram maior influência sobre a citotoxicidade do que sobre a resistência à flexão.


Subject(s)
Animals , Mice , Acrylic Resins/pharmacology , Dental Polishing , Acrylic Resins/toxicity , Cell Line , Materials Testing
14.
Braz. dent. j ; 24(4): 410-414, July-Aug/2013. tab, graf
Article in English | LILACS | ID: lil-689833

ABSTRACT

The aim of this study was to test the null hypothesis that there is no difference in fluoride release between resin-modified glass ionomer cements (RMGICs) and composites in the long term. The materials were divided into 5 groups: a nonfluoride-releasing composite - Group TXT (Transbond XT), a fluoride-releasing composite - Group QC (Quick-Cure), and three RMGICs - Groups FOLC, FOB and MC (Fuji Ortho LC, Fuji Ortho Band, and Multi-Cure). Fluoride release was measured at time intervals of 1 h, 1, 7, 14, 21 and 29 days, followed by further evaluations performed at 6, 12, 18, 24 and 30 months using selective ion electrodes connected to an ionic analyzer. Fluoride releasing and re-releasing experiments were analyzed using the Kruskal-Wallis test and Mann-Whitney test with the Bonferroni correction. The amount of fluoride released by FOB was larger in comparison with the other adhesives (p=0.01). In the long-term, FOLC and MC had a similar performance (p>0.05). The composites presented a low fluoride release, but fluoride ion uptake and re-release capacity of QC was statistically significant (p<0.05) during the experiment. In conclusion, the null hypothesis was rejected, the RMGIC Fuji Ortho Band and the composite Quick-Cure presented greater fluoride release and re-release capacity when recharged.


O objetivo deste estudo foi testar a hipótese nula de que não há nenhuma diferença de liberação de flúor entre cimentos de ionômero de vidro reforçados com resina (CIVRRs) e entre compósitos em longo prazo. Os materiais foram divididos em 5 grupos: um compósito não liberador de flúor - Grupo TXT (Transbond XT), um compósito liberador de flúor - Grupo QC (Quick-Cure), e três CIVRRs - Grupos FOLC, FOB e MC (Fuji Ortho LC, Fuji Ortho Band e Multi-Cure). A liberação de flúor foi medida em intervalos de tempo de 1 h, 1, 7, 14, 21 e 29 dias, seguido por outras avaliações realizadas aos 6, 12, 18, 24 e 30 meses, utilizando um eletrodo íon seletivo conectado a um analisador de íons. Liberação de flúor e experimentos de re-liberação foram avaliados usando o teste de Kruskal-Wallis e teste Mann-Whitney com correção de Bonferroni. A quantidade de flúor liberado pelo FOB foi maior em comparação com os outros adesivos (p=0,001). Em longo prazo, FOLC e MC demonstraram um desempenho semelhante (p>0,05). Os compósitos liberaram uma baixa quantidade de fluoreto, no entanto, a capacidade de captação do íon fluoreto e re-liberação do QC foi estatisticamente significante (p<0,05) durante o experimento. Em conclusão, a hipótese nula foi rejeitada, o CIVRR Fuji Ortho Band e o compósito Quick-Cure apresentaram maior liberação e capacidade de re-liberação de flúor quando recarregados.


Subject(s)
Humans , Dental Cements/chemistry , Fluorides/chemistry , Longitudinal Studies
15.
Dental press j. orthod. (Impr.) ; 18(3): 35-38, May-June 2013. graf, tab
Article in English | LILACS | ID: lil-689995

ABSTRACT

OBJECTIVE: The objective of this study was to test the hypothesis that there is no difference between stainless steel and low-nickel stainless steel wires as regards mechanical behavior. Force, resilience, and elastic modulus produced by Quad-helix appliances made of 0.032-inch and 0.036-inch wires were evaluated. METHODS: Sixty Quad-helix appliances were made, thirty for each type of alloy, being fifteen for each wire thickness, 0.032-in and 0.036-in. All the archwires were submitted to mechanical compression test using an EMIC DL-10000 machine simulating activations of 4, 6, 9, and 12 mm. Analysis of variance (ANOVA) with multiple comparisons and Tukey's test were used (p < 0.05) to assess force, resilience, and elastic modulus. RESULTS: Statistically significant difference in the forces generated, resilience and elastic modulus were found between the 0.032-in 0.036-in thicknesses (p < 0.05). CONCLUSIONS: Appliances made of low-nickel stainless steel alloy had force, resilience, and elastic modulus similar to those made of stainless steel alloy.


OBJETIVO: o objetivo do presente estudo foi testar a hipótese de não haver diferença no desempenho mecânico entre fios de aço inoxidável e fios de aço inoxidável com baixo teor de níquel. Avaliar, também, a força, a resiliência e o módulo de elasticidade produzidos pelos aparelhos quadri-hélice confeccionados com fio de 0,032" e 0,036". MÉTODOS: foram confeccionados 60 aparelhos quadri-hélice. Desses, 30 para cada tipo de liga, sendo 15 confeccionados com fio de 0,032" e 15 com fio 0,036". Todos os arcos foram submetidos a teste de compressão mecânica em máquina EMIC DL-10000, simulando 4, 6, 9, e 12mm de ativação. A análise de variância e comparação múltipla (ANOVA) e o teste de Tukey foram utilizados (p < 0,05) para avaliação da força, resiliência e módulo de elasticidade. RESULTADOS: observou-se diferença estatisticamente significativa entre forças produzidas, resiliência e módulo de elasticidade entre as espessuras 0,032" e 0,036" (p < 0,05). CONCLUSÃO: os aparelhos confeccionados com liga de aço inoxidável com baixo teor de níquel apresentaram liberação de força, resiliência e módulo de elasticidade semelhantes aos aparelhos que utilizaram a liga de aço inoxidável.


Subject(s)
Humans , Dental Alloys , Malocclusion/therapy , Orthodontic Appliance Design , Orthodontic Wires , Stainless Steel/chemistry , Analysis of Variance , Compressive Strength , Dental Stress Analysis , Elasticity , Materials Testing , Nickel , Stress, Mechanical
16.
Dental press j. orthod. (Impr.) ; 18(2): 101-107, Mar.-Apr. 2013. ilus, tab
Article in English | LILACS | ID: lil-683191

ABSTRACT

OBJECTIVE: To mechanically evaluate different systems used for incisors retraction. METHODS: Three different methods for incisors retraction using 0.019 x 0.025-in stainless steel wire were evaluated. The samples were divided into three groups: Group A (retraction arch with 7-mm high vertical hooks); Group G3 (elastic chain attached to the miniimplant and to the 3-mm stainless steel hook soldered to the retraction arch); Group G6 (elastic chain attached to the mini-implant and to the 6-mm stainless steel hook soldered to the retraction arch). A dental mannequin was used for evaluation in order to simulate the desired movements when the device was exposed to a heat source. The analysis of variance (ANOVA) and the Tukey test were used (p < 0.05). RESULTS: The results demonstrated that Groups G3 and G6 exhibited less extrusion and less incisor inclination during the retraction phase (p < 0.05). With regard to incisor extrusion, statistically significant differences were observed between Groups A and G3, and between Groups A and G6 (p < 0.05). Regarding incisor inclination, statistically significant differences were observed between the three systems evaluated (p < 0.05). CONCLUSIONS: Arches with 6-mm vertical hooks allow the force to be applied on the center of resistance of the incisors, thus improving mechanical control when compared with the other two systems.


OBJETIVO: avaliar mecanicamente diferentes sistemas utilizados para retração de incisivos. MÉTODOS: três diferentes métodos de retração dos incisivos foram avaliados, utilizando arco ortodôntico de retração confeccionado com fio de aço inoxidável 0,019" x 0,025" de espessura. Os grupos foram divididos em: Grupo A (arco de retração com alças verticais de 7mm de altura), Grupo G3 (elástico em cadeia ligado do mini-implante ao gancho de aço inoxidável com 3mm de altura soldado no arco de retração) e Grupo G6 (elástico em cadeia ligado do mini-implante ao gancho de aço inoxidável com 6mm de altura soldado no arco de retração). Para essa avaliação, adequou-se um manequim odontológico de tal forma que possibilitasse simular os movimentos desejados, quando fosse exposto a uma fonte de calor. Após obtenção das medidas dos movimentos, realizou-se análise estatística. RESULTADOS: os resultados demonstraram que os grupos G3 e G6 propiciaram menor extrusão e menor inclinação palatina dos incisivos na fase de retração (p<0,05). Quanto à extrusão dos incisivos, houve diferença estatisticamente significativa entre os grupos A e G3, e A e G6 (p<0,05). Em relação à inclinação, ocorreu diferença estatisticamente significativa entre os três sistemas avaliados (p<0,05). CONCLUSÃO: arcos com ganchos verticais de 6mm soldados permitem aproximar a linha de ação da força ao centro de resistência dos incisivos, proporcionando melhor controle mecânico, se comparado aos outros dois sistemas.


Subject(s)
Humans , Dental Implants , Incisor/pathology , Malocclusion/therapy , Maxilla/pathology , Orthodontic Wires , Orthodontic Anchorage Procedures/instrumentation , Tooth Movement Techniques/instrumentation , Analysis of Variance , Manikins , Orthodontic Anchorage Procedures/methods , Tooth Movement Techniques/methods
17.
Dental press j. orthod. (Impr.) ; 18(1): 45-49, Jan.-Feb. 2013. ilus
Article in English | LILACS | ID: lil-674262

ABSTRACT

INTRODUCTION: Since the beginning of miniscrews as orthodontic anchorage, many applications have been described in the literature. Among these, one is the uprighting of mesially inclined molars. In regard to the mechanical aspects, however, there is little information about the application of orthodontic forces using such devices. OBJECTIVE: The objective of this study was to describe a miniscrew supported spring for uprighting of mesially inclined molars. With this device, one can achieve the correct use of orthodontic biomechanics, thus favoring more predictable tooth movements and preventing unwanted movements from occurring.


INTRODUÇÃO: desde o surgimento dos mini-implantes como recurso de ancoragem ortodôntica, muitas aplicações têm sido descritas na literatura. Entre essas, cita-se a verticalização de molares inclinados para mesial. No entanto, pouco se fala da correta aplicação das forças ortodônticas nesses dispositivos, sob o ponto de vista mecânico. OBJETIVOS: o objetivo desse artigo foi demonstrar uma mola mini-implante suportada para verticalizar molares inclinados para mesial. Com esse dispositivo consegue-se correta aplicação da mecânica ortodôntica, favorecendo movimentos mais previsíveis e minimizando os movimentos indesejáveis.


Subject(s)
Female , Humans , Male , Bone Screws , Molar/abnormalities , Orthodontic Anchorage Procedures/instrumentation , Tooth Movement Techniques/instrumentation , Tooth Root/physiology , Dental Stress Analysis , Tooth Movement Techniques/methods , Tooth Root
18.
Rev. odontol. Univ. Cid. São Paulo (Online) ; 24(3)set.-dez. 2012. tab, graf
Article in Portuguese | LILACS, BBO | ID: lil-661327

ABSTRACT

Introdução: O objetivo deste estudo é testar a hipótese de que não há diferença no desempenho de compósitos ortodônticos entre si quanto à liberação de flúor. Métodos: Os materiais foram divididos em 3 grupos: 2 compósitos ortodônticos utilizados para colagem de bráquetes ortodônticos: Grupo SI (SuperBond Instant, OrthoSource) e Grupo CC (Color Change, OrthoSource) e 1 cimento de ionômero de vidro (CIV) convencional utilizado para cimentação de bandas ortodônticas; Grupo S (Sumo, OrthoSource), como controle. A liberação de flúor foi medida durante 28 dias (1h, 24h, 1, 3, 7, 14, 21 e 28 dias), através de eletrodo íon seletivo conectado a um analisador de íons. Os dados foram submetidos aos testes não paramétricos de Kruskal-Wallis e de Mann-Whitney, a um nível de significância de 5%. Resultados: Os resultados evidenciaram que os materiais atingiram o pico máximo de liberação de flúor com 24h após presa inicial. Houve diferença estatística entre os grupos SI e CC com o grupo S em todos os tempos avaliados (p < 0.05). Houve diferença estatística entre os grupos SI e CC nos tempos de 1h, 24h, 3, 7, 14 e 21 dias (p < 0.05). Conclusão: A hipótese foi rejeitada. O compósito Color Change apresentou desempenho melhor comparado ao SuperBond Instant, porém ambos apresentaram uma liberação de flúor pouco expressiva comparados ao CIV convencional.


Introduction: The objective of this study is to test the hypothesis that there is no difference in performance of orthodonticcomposites in terms of their fluoride release. Methods: The materials were divided into three groups:two composites used for orthodontic Bonding of orthodontic brackets: Group SI (Instant SuperBond, Ortho-Source) and Group CC (Color Change, OrthoSource) and a glass ionomer cement (GIC) used for conventionalcementing orthodontic bands: Group S (Sumo, OrthoSource) as the control. Fluoride release was measuredduring 28 Days (1h, 24h, 1, 3, 7, 14, 21 and 28 Days), by ion selective electrode connected to an ion analyzer.The data were subjected to nonparametric tests of Kruskal-Wallis and Mann-Whitney, with a significance levelof 5%. Results: The results showed that the materials reached the peak of fluoride release after 24 hours afterinitial set. Statistical difference between groups SI and DC with the group S at all times evaluated (p <0.05).Statistical difference between groups SI and CC in 1h, 24h, 3, 7, 14 and 21 Days (p <0.05). Conclusion: Thehypothesis was rejected. Color Change composite performance was better compared to SuperBond Instant, butboth had a very expressive fluoride release compared to conventional GIC.


Subject(s)
Compomers , Fluorine , Glass Ionomer Cements
19.
Dental press j. orthod. (Impr.) ; 17(6): 21e1-21e5, Nov.-Dec. 2012. graf, tab
Article in English | LILACS | ID: lil-669386

ABSTRACT

OBJECTIVE: To evaluate the cytotoxicity of three different alginate impression materials for orthodontic use. METHODS: Three different brands of alginate were divided into three groups, namely, Group JCO (Jeltrate Chromatic Ortho), OP (Orthoprint) and CO (Cavex Orthotrace). Three control groups were also included: Group C+ (positive control), consisting of detergent Tween 80; Group C- (negative control), consisting of PBS, and Group CC (cell control), consisting of cells not exposed to any material. After manipulating the materials according to the respective manufacturer instructions, samples were made with the use of silicon rings. Then the samples were immersed in Eagle's minimum essential medium (MEM) for 2 minutes. The supernatants were then removed and brought into direct contact with L929 fibroblasts. After exposure to the medium, the cells were incubated for 24 hours. Then 100 µl of 0.01% neutral red dye were added. The cells were incubated again for 3 hours so that the dye could be absorbed. After this 3-hour period, the cells were fixed to perform the viable cell count, using a spectrophotometer (BioTek, Winooski, Vermont, USA) at a wavelength of 492 nm. RESULTS: Statistical differences were found when Groups CC and C- were compared with the other experimental groups. Group JCO had the highest cytotoxicity, followed by Groups OP and CO. CONCLUSION: Based on the results obtained in this work, it was concluded that all alginate impression materials are potentially cytotoxic.


OBJETIVO: avaliar a citotoxicidade de três diferentes alginatos de uso ortodôntico. MÉTODOS: foram avaliados três diferentes alginatos divididos em três grupos, denominados grupo JCO (Jeltrate Chromatic Ortho), OP (Orthoprint) e CO (Carrex Orthotrace). Três grupos controle também participaram: controle + (C+), constituído pelo detergente celular Tween 80; controle - (C-) PBS; e controle de célula (CC) onde as células não foram expostas a nenhum material. Após manipulação dos materiais, seguindo as orientações do fabricante, foram confeccionados corpos de prova utilizando-se anéis de silicone. Em seguida, esses foram imersos em meio mínimo essencial de Eagle (MEM) por 2min, onde, então, procedeu-se à remoção do sobrenadante e à colocação em contato com fibroblastos L929. Após contato com o meio, as células foram incubadas por mais 24h onde, então, foi adicionado o corante vermelho neutro a 0,01%. Passado esse período, foram fixadas e, então, realizada contagem de células viáveis em espectrofotômetro (BioTek, Vermont, EUA) em um comprimento de onda de 492nm. RESULTADOS: os resultados demonstraram diferenças estatística entre os grupos CC e C- com os demais. O grupo experimental JCO mostrou-se com maior citotoxicidade, seguido pelos grupso OP e CO. CONCLUSÕES: pode-se concluir, com a realização desse trabalho, que todos os alginatos testados mostraram caráter citotóxico.

20.
Full dent. sci ; 4(13): 150-153, out.-dez. 2012. tab
Article in Portuguese | LILACS, BBO | ID: lil-681687

ABSTRACT

O objetivo deste trabalho foi avaliar a citotoxicidade do enxaguatório bucal Plax Tradicional. Avaliou-se em diferentes tempos: 1, 15, 30, 45, 60 e 120 segundos quanto seu efeito citotóxico em fibroblastos gengivais L929. Utilizou-se 3 grupos controle: positivo (C+) detergente celular Tween 80, negativo (C-) PBS, e controle de célula (CC) onde as células não foram expostas a nenhum material. O ensaio de citotoxicidade foi realizado utilizando cultura celular de fibroblasto de camundongo (L929). Após contato do enxaguatório com as células, as mesmas foram colocadas em contato com o corante vital vermelho neutro utilizando-se a técnica “dye uptake”. Os valores da quantidade de células viáveis foram submetidos à análise da variância (ANOVA) para determinar se havia diferença estatística entre os grupos, e posteriormente, ao teste de Tukey (p<0.05). Os resultados demonstraram citotoxicidade do enxaguatório com diferenças estatísticas para os grupos CC e C- (p<0.05). Em relação ao controle positivo, o Plax foi mais citotóxico nos tempos 45, 60 e 120 segundos. A citotoxicidade foi diretamente proporcional ao tempo de exposição às culturas de células. Dessa forma, pode- -se concluir que o enxaguatório Plax tradicional é altamente citotóxico a fibroblastos gengivais


The aim of this study was to evaluate the cytotoxicity of Traditional Plax mouthwash. It was evaluated at different times: 1, 15, 30, 45, 60, and 120 seconds as their cytotoxic effect on gingival fibroblasts L929. We used three control groups: positive (C +) cell detergent Tween 80 was negative (C-), PBS, and cell control (CC) where the cells were not exposed to any material. The cytotoxicity assay was performed using cell culture of mouse fibroblast (L929). After contacting the mouthwash with the cells, they were placed in contact with the vital dye neutral red using the technique (dye uptake). The values ​of the amount of viable cells were subjected to analysis of variance (ANOVA) to determine whether there were statistical differences between groups, and subsequently Tukey test (p <0.05). The results showed cytotoxicity of mouthwash with statistical differences for the CC and C- (p <0.05). In relation to the positive control, Plax was more cytotoxic in the days 45, 60, and 120 seconds. Cytotoxicity was directly proportional to exposure time to cell cultures. Thus we can conclude that the traditional Plax mouthwash is highly cytotoxic to gingival fibroblasts


Subject(s)
Mouthwashes/administration & dosage , Fibroblasts/pathology , In Vitro Techniques , Oral and Dental Hygiene Products , Cell Culture Techniques , Analysis of Variance
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